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Dernière mise à jour : Mai 2018

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Human Nutrition Unit

Zone de texte éditable et éditée et rééditée

Viral vectors

Tools for in vivo and in vitro modulation of gene expression

We use and/or build virals vectors in order to over- or -underexpress genes of interest in vitro and in vivo :

• lentiviral vectors (LV)

• adeno-associated vectors (AAV)

• adenoviral vectors (Ad) 

Exemples :

In vitro

In vivo

BHLHE40 transcription factor overexpression in muscle cells induced myotube atrophy.                                                                                                                                    

Lecomte et al. Mol Cel Biol 2010

Mouse muscle overexpression of GFP/ SREBP-1 transcription factor. Muscle histology revealed fiber size reduction.                                                                                                           

Lecomte et al. Mol Cel Biol 2010

Injection of LV-AARE-LUC in mouse pancreas. Luciferase expression is under the control of an artificial promoter based on AARE sequences and –Ile is the inducer (see NUTRIREG).

Chaveroux et al, Nature Biotech. 2016

General informations :

As well as viruses, viral vectors are able to enter into and to genetically modify targed cells. Cellular and tissular specificity depend on serotype. Besides, genetic modification could be stable or transient, according to kind of viral vector.

Viral vectors could (1) express a transgene of interest, or (2) express a shRNA in order to shut down transgene of interest expression. Unlike viruses, viral vectors are devoid of their replication ability.

Contact : 

Cyrielle VITURET