En savoir plus

18 months post-doc position in PIAF laboratory, Clermont-Ferrand, France

Monitor eATP-induced [Ca2+]cytdynamics in Arabidopsis root tip

Official start date: September 01st 2019 (dead-line to apply: June 30th 2019).

The project is a collaborative ERA-CAPS project between the MECA group of the PIAF, the group of Julia Davis (University of Cambridge, UK) and the group of Gary Stacey (University of Missouri, USA).
 

All known organisms use adenosine 5ʹ-triphosphate (ATP) as an essential, cellular energy source to drive many biochemical reactions. ATP can also be released into the extracellular matrix, where it is referred to as extracellular ATP (eATP). Numerous studies showed that eATPhas been implicated in a variety of plant processes, including roothair growth, gravitropism, pathogen responses and thig-motropism(Werasinghe et al., 2009). DORN1-a lectin receptor-like kinase-has been identified as the primary eATP receptor in Arabidopsis (Choi et al., 2014; Cao et al. 2014). In parallel, studies showed extracellular ATP signaling is associated with an elevation of cytoplasmic calcium [Ca2+]cyt.andaDORN1-mediated activation of plasma membrane K+-and Ca2+permeable conduct-ances (Wang et al., 2018). In this project, we are exploring the role that DORN1-mediated eATP signalling plays in mechano-responses. We will determine the effect of impaired DORN1on the stem response to wind and the root response to changes in soili mpedance. The eATP-induced [Ca2+]cyt dynamics at cellular level, will monitor in root tip and in response to mechanical stimulus using sensitive GECO [Ca2+]cytsen-sor (Waadt et al., 2017;Keinath et al., 2015). An original vertical stage fluorescent microscope and/or a Light Sheet Microscope will be used to follow [Ca2+]cyt dynamics.